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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TLR8 Monoclonal antibody

TLR8 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3B6A4

Cat no : 67317-1-Ig

Synonyms

CD288, TLR8, toll like receptor 8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TLR8 Monoclonal antibody (67317-1-Ig)

    WB analysis using 67317-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67317-1-Ig (TLR8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using TLR8 Monoclonal antibody (67317-1-Ig)

    WB analysis of U-937 using 67317-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 67317-1-Ig (TLR8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TLR8 Monoclonal antibody (67317-1-Ig)

    IHC staining of human tonsillitis using 67317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TLR8 Monoclonal antibody (67317-1-Ig)

    IHC staining of human tonsillitis using 67317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using TLR8 Monoclonal antibody (67317-1-Ig)

    IF Staining of human tonsillitis using 67317-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using TLR8 Monoclonal antibody (67317-1-Ig)

    IF Staining of human tonsillitis using 67317-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inDaudi cells, U-937 cells, K-562 cells, HL-60 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67317-1-Ig targets TLR8 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TLR8 fusion protein Ag16566 相同性解析による交差性が予測される生物種
Full Name toll-like receptor 8
Calculated molecular weight 1041 aa, 120 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC101075
Gene symbol TLR8
Gene ID (NCBI) 51311
RRIDAB_2882577
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses (PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms,120 and 122 kDa.

Protocols

Product Specific Protocols
WB protocol for TLR8 antibody 67317-1-IgDownload protocol
IHC protocol for TLR8 antibody 67317-1-IgDownload protocol
IF protocol for TLR8 antibody 67317-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols