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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated TLR8 Monoclonal antibody

TLR8 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B6A4

Cat no : CL488-67317

Synonyms

CD288, TLR8, toll like receptor 8

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated TLR8 Monoclonal anti (CL488-67317)

    IF Staining of human tonsillitis using CL488-67317

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 TLR8 antibody (CL488-67317, Clone: 3B6A4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated TLR8 Monoclonal anti (CL488-67317)

    IF Staining of human tonsillitis using CL488-67317

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 TLR8 antibody (CL488-67317, Clone: 3B6A4 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67317 targets TLR8 in IF-P applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TLR8 fusion protein Ag16566 相同性解析による交差性が予測される生物種
Full Name toll-like receptor 8
Calculated molecular weight 1041 aa, 120 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC101075
Gene symbol TLR8
Gene ID (NCBI) 51311
RRIDAB_2919451
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses (PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms,120 and 122 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TLR8 antibody CL488-67317Download protocol
Standard Protocols
Click here to view our Standard Protocols