CoraLite® Plus 488-conjugated TLR8 Monoclonal antibody
TLR8 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3B6A4
Cat no : CL488-67317
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-67317 targets TLR8 in IF-P applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | TLR8 fusion protein Ag16566 相同性解析による交差性が予測される生物種 |
Full Name | toll-like receptor 8 |
Calculated molecular weight | 1041 aa, 120 kDa |
Observed molecular weight | 120 kDa |
GenBank accession number | BC101075 |
Gene symbol | TLR8 |
Gene ID (NCBI) | 51311 |
RRID | AB_2919451 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses (PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms,120 and 122 kDa.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 TLR8 antibody CL488-67317 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |