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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMED10 Monoclonal antibody, PBS Only

TMED10 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3B11F9

Cat no : 67876-1-PBS

Synonyms

P24(DELTA), p24delta, S31I125, S31III125, TMED10, Tmp 21 I, TMP21, Transmembrane protein Tmp21

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMED10 Monoclonal antibody (67876-1-Ig)

    WB analysis using 67876-1-Ig (same clone as 67876-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMED10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMED10 Monoclonal antibody (67876-1-Ig)

    WB analysis using 67876-1-Ig (same clone as 67876-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMED10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TMED10 Monoclonal antibody (67876-1-Ig)

    IHC staining of human colon cancer using 67876-1-Ig (same clone as 67876-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMED10 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TMED10 Monoclonal antibody (67876-1-Ig)

    IHC staining of human colon cancer using 67876-1-Ig (same clone as 67876-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMED10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMED10 Monoclonal antibody (67876-1-Ig)

    IHC staining of mouse brain using 67876-1-Ig (same clone as 67876-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMED10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMED10 Monoclonal antibody (67876-1-Ig)

    IHC staining of mouse brain using 67876-1-Ig (same clone as 67876-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMED10 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMED10 Monoclonal antibody (67876-1-Ig)

    IF Staining of HeLa using 67876-1-Ig (same clone as 67876-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TMED10 antibody (67876-1-Ig, Clone: 3B11F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67876-1-PBS targets TMED10 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TMED10 fusion protein Ag7516 相同性解析による交差性が予測される生物種
Full Name transmembrane emp24-like trafficking protein 10 (yeast)
Calculated molecular weight 25 kDa
Observed molecular weight21 kDa
GenBank accession numberBC001825
Gene symbol TMED10
Gene ID (NCBI) 10972
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.