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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMP21 Monoclonal antibody

TMP21 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3B11F9

Cat no : 67876-1-Ig

Synonyms

TMED10, Protein TMED10, p24delta1, p24 family protein delta-1, p23

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMP21 Monoclonal antibody (67876-1-Ig)

    WB analysis using 67876-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMP21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using TMP21 Monoclonal antibody (67876-1-Ig)

    WB analysis using 67876-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMP21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TMP21 Monoclonal antibody (67876-1-Ig)

    IHC staining of human colon cancer using 67876-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TMP21 Monoclonal antibody (67876-1-Ig)

    IHC staining of human colon cancer using 67876-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMP21 Monoclonal antibody (67876-1-Ig)

    IHC staining of mouse brain using 67876-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMP21 Monoclonal antibody (67876-1-Ig)

    IHC staining of mouse brain using 67876-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMP21 Monoclonal antibody (67876-1-Ig)

    IF Staining of HeLa using 67876-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TMP21 antibody (67876-1-Ig, Clone: 3B11F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using TMP21 Monoclonal antibody (67876-1-Ig)

    FC experiment of HeLa using 67876-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug TMP21 Monoclonal antibody (67876-1-Ig, Clone:3B11F9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA549 cells, pig liver tissue, HeLa cells, HepG2 cells, Jurkat cells, Raji cells, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67876-1-Ig targets TMP21 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TMP21 fusion protein Ag7516 相同性解析による交差性が予測される生物種
Full Name transmembrane emp24-like trafficking protein 10 (yeast)
Calculated molecular weight 25 kDa
Observed molecular weight21 kDa
GenBank accession numberBC001825
Gene symbol TMED10
Gene ID (NCBI) 10972
RRIDAB_2918633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMP21 antibody 67876-1-IgDownload protocol
IHC protocol for TMP21 antibody 67876-1-IgDownload protocol
IF protocol for TMP21 antibody 67876-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols