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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated TMP21 Monoclonal antibody (CL594-67876)

    FC experiment of HeLa using CL594-67876

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TMP21 (CL594-67876, Clone:3B11F9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite®594-conjugated TMP21 Monoclonal antibody

TMP21 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67876
Clone No.3B11F9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

FC (Intra)

Synonyms

TMED10, Protein TMED10, p24delta1, p24 family protein delta-1, p23

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67876 targets TMP21 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TMP21 fusion protein Ag7516 相同性解析による交差性が予測される生物種
Full Name transmembrane emp24-like trafficking protein 10 (yeast)
Calculated molecular weight 25 kDa
Observed molecular weight21 kDa
GenBank accession numberBC001825
Gene Symbol TMED10
Gene ID (NCBI) 10972
RRIDAB_2923929
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49755
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL594 TMP21 antibody CL594-67876Download protocol
Standard Protocols
Click here to view our Standard Protocols