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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated TMEM119 Polyclonal antibody

TMEM119 Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-27585

Synonyms

UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CoraLite® Plus 647-conjugated TMEM119 Polyclonal a (CL647-27585)

    IF Staining of mouse liver using CL647-27585

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CoraLite® Plus 647 TMEM119 antibody (CL647-27585) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 647-conjugated TMEM119 Polyclonal a (CL647-27585)

    FC experiment of HEK-293 using CL647-27585

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated TMEM119 Polyclonal antibody (CL647-27585)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse liver tissue
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-27585 targets TMEM119 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM119 fusion protein Ag26269 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 119
Calculated molecular weight 29 kDa
Observed molecular weight 45 kDa
GenBank accession numberNM_181724
Gene symbol TMEM119
Gene ID (NCBI) 338773
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#27585-1-AP. The predicted MW of TMEM119 is 29 kDa. Catalog#27585-1-AP recognizes 45-50 kDa band may due to glycosylation..

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 TMEM119 antibody CL647-27585Download protocol
FC protocol for CL Plus 647 TMEM119 antibody CL647-27585Download protocol
Standard Protocols
Click here to view our Standard Protocols