Validation Data Gallery

Filter: AllWBIPIHCIF-PIF-FroIF/ICCFC (Intra)

WB analysis of THP-1 using 19851-1-AP
WB result of TMEM173/STING antibody (19851-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells.
WB analysis of HL-60 using 19851-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 19851-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 19851-1-AP
WB result of TMEM173/STING antibody (19851-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and TMEM173/STING knockout HepG2 cells.
WB analysis using 19851-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 19851-1-AP
WB result of TMEM173/STING antibody (19851-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected HEK-293 cells.
WB analysis of Raji using 19851-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 19851-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 19851-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of mouse spleen using 19851-1-AP
IP result of anti-TMEM173/STING (IP:19851-1-AP, 4ug; Detection:19851-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.
IHC staining of human tonsillitis using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse thymus using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse thymus using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat thymus using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat thymus using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human spleen using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human spleen using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 19851-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 19851-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human tonsillitis using 19851-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 19851-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HT-29 using 19851-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of THP-1 using 19851-1-AP
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TMEM173/STING antibody (19851-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of THP-1 using 19851-1-AP
1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TMEM173/STING (19851-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Cat No. 19851-1-AP

Publications(453)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA, Blocking assay

Synonyms

STING, Endoplasmic reticulum interferon stimulator, ERIS, MITA, MPYS

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (453)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, HEK-293 cells, HepG2 cells, mouse kidney tissue, mouse spleen tissue, Raji cells, THP-1 cells, HT-29 cells, mouse thymus tissue, C2C12 cells, RAW 264.7 cells, mouse testis tissue
Positive IP detected in	mouse spleen tissue
Positive IHC detected in	human tonsillitis tissue, rat thymus tissue, mouse thymus tissue, human spleen tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue
Positive IF-Fro detected in	mouse brain tissue
Positive IF/ICC detected in	HT-29 cells, human tonsillitis tissue, THP-1 cells
Positive FC (Intra) detected in	THP-1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 50 publications below
WB	See 381 publications below
IHC	See 70 publications below
IF	See 127 publications below
IP	See 13 publications below
CoIP	See 9 publications below

Product Information

19851-1-AP targets TMEM173/STING in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, bovine, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13921 Product name: Recombinant human TMEM173 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 174-379 aa of BC047779 Sequence: ELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS 相同性解析による交差性が予測される生物種
Full Name	transmembrane protein 173
Calculated molecular weight	379 aa, 42 kDa
Observed molecular weight	35-40 kDa, 80 kDa
GenBank accession number	BC047779
Gene Symbol	STING
Gene ID (NCBI)	340061
RRID	AB_10665370
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86WV6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

Protocols

Product Specific Protocols
IF protocol for TMEM173/STING antibody 19851-1-AP	Download protocol
IHC protocol for TMEM173/STING antibody 19851-1-AP	Download protocol
IP protocol for TMEM173/STING antibody 19851-1-AP	Download protocol
WB protocol for TMEM173/STING antibody 19851-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins Authors - Huaqing Deng View Article
mouse	WB	Nature eccDNAs are apoptotic products with high innate immunostimulatory activity Authors - Yuangao Wang KO Validated View Article
mouse	IF	Nature Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C. Authors - Ting-Ting Chu KO Validated View Article
human	WB	Science The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling. Authors - Mena Abdel-Nour View Article
human	WB,IF	Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion Authors - Wenwen Zheng KD Validated View Article
human	WB	Cancer Cell Mutant p53 suppresses innate immune signaling to promote tumorigenesis. Authors - Monisankar Ghosh KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of THP-1 using 19851-1-AP

WB analysis of HL-60 using 19851-1-AP

WB analysis using 19851-1-AP

WB analysis of HepG2 using 19851-1-AP

WB analysis using 19851-1-AP

WB analysis of HEK-293 using 19851-1-AP

WB analysis of Raji using 19851-1-AP

WB analysis of mouse spleen using 19851-1-AP

WB analysis using 19851-1-AP

IP experiment of mouse spleen using 19851-1-AP

IHC staining of human tonsillitis using 19851-1-AP

IHC staining of human tonsillitis using 19851-1-AP

IHC staining of mouse thymus using 19851-1-AP

IHC staining of mouse thymus using 19851-1-AP

IHC staining of human liver cancer using 19851-1-AP

IHC staining of human liver cancer using 19851-1-AP

IHC staining of rat thymus using 19851-1-AP

IHC staining of rat thymus using 19851-1-AP

IHC staining of human spleen using 19851-1-AP

IHC staining of human spleen using 19851-1-AP

IHC staining of human tonsillitis using 19851-1-AP

IF Staining of human tonsillitis using 19851-1-AP

IF Staining of human tonsillitis using 19851-1-AP

IF Staining of mouse brain using 19851-1-AP

IF Staining of HT-29 using 19851-1-AP

IF Staining of THP-1 using 19851-1-AP

FC experiment of THP-1 using 19851-1-AP

TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins

eccDNAs are apoptotic products with high innate immunostimulatory activity

Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C.

The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling.

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Mutant p53 suppresses innate immune signaling to promote tumorigenesis.