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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19851

Synonyms

STING, NET23, MPYS, MITA, ERIS

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CoraLite® Plus 488-conjugated TMEM173/STING Polycl (CL488-19851)

    IF Staining of HT-29 using CL488-19851

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite® Plus 488 TMEM173/STING antibody (CL488-19851) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated TMEM173/STING Polycl (CL488-19851)

    IF Staining of HepG2 using CL488-19851

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated TMEM173 antibody (CL488-19851) at dilution of 1:400.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated TMEM173/STING Polycl (CL488-19851)

    FC experiment of HepG2 using CL488-19851

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TMEM173/STING (CL488-19851) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHT-29 cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19851 targets TMEM173/STING in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM173/STING fusion protein Ag13921 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 173
Calculated molecular weight 379 aa, 42 kDa
GenBank accession numberBC047779
Gene symbol STING
Gene ID (NCBI) 340061
RRIDAB_2883086
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TMEM173/STING antibody CL488-19851Download protocol
FC protocol for CL Plus 488 TMEM173/STING antibody CL488-19851Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Life Sci

Icariside II alleviates lipopolysaccharide-induced acute lung injury by inhibiting lung epithelial inflammatory and immune responses mediated by neutrophil extracellular traps

Authors - Xiuchun Li
View Article