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Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    WB analysis of THP-1 using 66680-1-Ig (same clone as 66680-1-PBS)

    WB result of TMEM173/STING antibody (66680-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    WB analysis using 66680-1-Ig (same clone as 66680-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    WB analysis of HSC-T6 using 66680-1-Ig (same clone as 66680-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    WB analysis of HEK-293 using 66680-1-Ig (same clone as 66680-1-PBS)

    WB result of TMEM173/STING antibody (66680-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected HEK-293 cells. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    WB analysis of HepG2 using 66680-1-Ig (same clone as 66680-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    IHC staining of human spleen using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    IHC staining of human spleen using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    IF Staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TMEM173/STING antibody (66680-1-Ig, Clone: 1F1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TMEM173/STING Monoclonal antibody, PBS Only

TMEM173/STING Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66680-1-PBS
Clone No.1F1E1

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

STING, NET23, MPYS, MITA, ERIS

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66680-1-PBS targets TMEM173/STING in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag20363

Product name: Recombinant human TMEM173 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 174-379 aa of BC047779

Sequence: ELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 173
Calculated molecular weight 379 aa, 42 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC047779
Gene Symbol STING
Gene ID (NCBI) 340061
RRIDAB_2882034
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ86WV6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).