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Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    WB analysis of THP-1 using 66680-1-Ig (same clone as 66680-1-PBS)

    WB result of TMEM173/STING antibody (66680-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    WB analysis using 66680-1-Ig (same clone as 66680-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    WB analysis of HSC-T6 using 66680-1-Ig (same clone as 66680-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    WB analysis of HEK-293 using 66680-1-Ig (same clone as 66680-1-PBS)

    WB result of TMEM173/STING antibody (66680-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected HEK-293 cells. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    WB analysis of HepG2 using 66680-1-Ig (same clone as 66680-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    IHC staining of human spleen using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    IHC staining of human spleen using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using TMEM173/STING Monoclonal antibody (66680-1-Ig)

    IF Staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TMEM173/STING antibody (66680-1-Ig, Clone: 1F1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TMEM173/STING Monoclonal antibody, PBS Only

TMEM173/STING Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66680-1-PBS
Clone No.1F1E1

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

STING, NET23, MPYS, MITA, ERIS

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66680-1-PBS targets TMEM173/STING in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TMEM173/STING fusion protein Ag20363 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 173
Calculated molecular weight 379 aa, 42 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC047779
Gene Symbol STING
Gene ID (NCBI) 340061
RRIDAB_2882034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86WV6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).