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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated TMEM53 Recombinant antibody

TMEM53 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240511C3

Cat no : CL488-83554-3

Synonyms

Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated TMEM53 Recombinant a (CL488-83554-3)

    IF Staining of A549 using CL488-83554-3

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 TMEM53 antibody (CL488-83554-3, Clone: 240511C3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated TMEM53 Recombinant a (CL488-83554-3)

    FC experiment of A549 using CL488-83554-3

    1x10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated TMEM53 Recombinant antibody (CL488-83554-3, Clone:240511C3)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83554-3 is TMEM53 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM53 fusion protein Ag21321 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 53
Calculated molecular weight 277 aa, 32 kDa
GenBank accession numberBC064520
Gene symbol TMEM53
Gene ID (NCBI) 79639
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM53 was initially described as a nuclear envelope transmembrane (NET) protein because of its nuclear envelope localization. Research has found that TMEM53 functionality does not rely on viral RNA binding or canonical IFN responses, and TMEM53 exerts an antiviral effect on other bat HKU2-related CoVs in a species-specific manner. Therefore, TMEM53 may be a potential therapeutic target for HKU2-related CoV infections and useful for preparing for future outbreaks of this viral species. (PMID: 37584551)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TMEM53 antibody CL488-83554-3Download protocol
FC protocol for CL Plus 488 TMEM53 antibody CL488-83554-3Download protocol
Standard Protocols
Click here to view our Standard Protocols