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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TMEM65 Recombinant antibody (83219-4-RR)

    WB analysis of HEK-293 using 83219-4-RR (same clone as 83219-4-PBS)

    WB result of TMEM65 antibody (83219-4-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM65 transfected HEK-293 cells. This data was developed using the same antibody clone with 83219-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM65 Recombinant antibody (83219-4-RR)

    WB analysis using 83219-4-RR (same clone as 83219-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83219-4-RR (TMEM65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83219-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM65 Recombinant antibody (83219-4-RR)

    WB analysis using 83219-4-RR (same clone as 83219-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83219-4-RR (TMEM65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83219-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83219-4-RR

    Affinity and Kinetic Characterization of 83219-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83219-4-RR against Human TMEM65 were performed. The affinity constant is 0.365 nM.

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  • Featured Product
  • KD/KO Validated

TMEM65 Recombinant antibody, PBS Only

TMEM65 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83219-4-PBS
Clone No.230512D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Transmembrane protein 65, 230512D7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83219-4-PBS targets TMEM65 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM65 fusion protein Ag15509 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 65
Calculated molecular weight 240 aa, 26 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC032396
Gene Symbol TMEM65
Gene ID (NCBI) 157378
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6PI78
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.