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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM65 Recombinant antibody

TMEM65 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230512D7

Cat no : 83219-4-RR

Synonyms

Transmembrane protein 65, 230512D7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM65 Recombinant antibody (83219-4-RR)

    WB analysis using 83219-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83219-4-RR (TMEM65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TMEM65 Recombinant antibody (83219-4-RR)

    WB analysis using 83219-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83219-4-RR (TMEM65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83219-4-RR

    Affinity and Kinetic Characterization of 83219-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83219-4-RR against Human TMEM65 were performed. The affinity constant is 0.365 nM.

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Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, PC-3 cells, SH-SY5Y cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83219-4-RR is TMEM65 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM65 fusion protein Ag15509 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 65
Calculated molecular weight 240 aa, 26 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC032396
Gene symbol TMEM65
Gene ID (NCBI) 157378
RRIDAB_3670904
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM65 antibody 83219-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols