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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMPO/LAP2 Monoclonal antibody

TMPO/LAP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3G4E1

Cat no : 67157-1-Ig

Synonyms

CMD1T, LAP2, LEMD4, PRO0868, Splenin Thymopentin, thymopoietin, Thymopoietin isoform alpha, TMPO, TMPO/LAP2, TP, TP alpha, TP5, TPRP isoform alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    WB analysis using 67157-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    WB analysis of SH-SY5Y using 67157-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    WB analysis of HSC-T6 using 67157-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IHC staining of rat brain using 67157-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IHC staining of human liver cancer using 67157-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IHC staining of human liver cancer using 67157-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IHC staining of rat brain using 67157-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IHC staining of mouse brain using 67157-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IHC staining of mouse brain using 67157-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IF Staining of HeLa using 67157-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (67157-1-Ig, Clone: 3G4E1 ) at dilution of 1:2200 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, K-562 cells
Positive IHC detected inrat brain tissue, human liver cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67157-1-Ig targets TMPO/LAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMPO/LAP2 fusion protein Ag6415 相同性解析による交差性が予測される生物種
Full Name thymopoietin
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa, 39 kDa
GenBank accession numberBC053675
Gene symbol TMPO/LAP2
Gene ID (NCBI) 7112
RRIDAB_2882454
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).

Protocols

Product Specific Protocols
WB protocol for TMPO/LAP2 antibody 67157-1-IgDownload protocol
IHC protocol for TMPO/LAP2 antibody 67157-1-IgDownload protocol
IF protocol for TMPO/LAP2 antibody 67157-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols