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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    WB analysis using 67157-1-Ig (same clone as 67157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    WB analysis of SH-SY5Y using 67157-1-Ig (same clone as 67157-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    WB analysis of HSC-T6 using 67157-1-Ig (same clone as 67157-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IHC staining of human liver cancer using 67157-1-Ig (same clone as 67157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IHC staining of rat brain using 67157-1-Ig (same clone as 67157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IHC staining of rat brain using 67157-1-Ig (same clone as 67157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IHC staining of human liver cancer using 67157-1-Ig (same clone as 67157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IHC staining of mouse brain using 67157-1-Ig (same clone as 67157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IHC staining of mouse brain using 67157-1-Ig (same clone as 67157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMPO/LAP2 Monoclonal antibody (67157-1-Ig)

    IF Staining of HeLa using 67157-1-Ig (same clone as 67157-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (67157-1-Ig, Clone: 3G4E1 ) at dilution of 1:2200 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67157-1-PBS in a different storage buffer formulation.

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TMPO/LAP2 Monoclonal antibody, PBS Only

TMPO/LAP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67157-1-PBS
Clone No.3G4E1

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CMD1T, LAP2, LEMD4, PRO0868, Splenin Thymopentin, thymopoietin, Thymopoietin isoform alpha, TMPO, TMPO/LAP2, TP, TP alpha, TP5, TPRP isoform alpha

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67157-1-PBS targets TMPO/LAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMPO/LAP2 fusion protein Ag6415 相同性解析による交差性が予測される生物種
Full Name thymopoietin
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa, 39 kDa
GenBank accession numberBC053675
Gene Symbol TMPO/LAP2
Gene ID (NCBI) 7112
RRIDAB_2882454
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP42167
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).