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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNF-alpha Recombinant antibody, PBS Only

TNF-alpha Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240668B4

Cat no : 83721-7-PBS

Synonyms

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using TNF-alpha Recombinant antibody (83721-7-RR)

    WB analysis of THP-1 using 83721-7-RR (same clone as 83721-7-PBS)

    Untreated, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 83721-7-RR (TNF-alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83721-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83721-7-RR

    Affinity and Kinetic Characterization of 83721-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83721-7-RR against Human TNF-alpha were performed. The affinity constant is 8.51 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83721-7-PBS is TNF-alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (TNF superfamily, member 2)
Calculated molecular weight26 kDa
GenBank accession numberNM_000594.4
Gene symbol TNF-alpha
Gene ID (NCBI) 7124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.