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TNF-alpha Recombinant antibody

TNF-alpha Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83721-7-RR
Clone No.240668B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  100 μL, 1000 ug/ml
20 μL, 1000 ug/ml
100 μL, 1000 ug/ml

¥85,000/ 100 μL

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Tested Applications

Positive WB detected inTHP-1 cells, LPS treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83721-7-RR targets TNF-alpha in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (TNF superfamily, member 2)
Calculated molecular weight26 kDa
Observed molecular weight26 kDa
GenBank accession numberNM_000594.4
Gene Symbol TNF-alpha
Gene ID (NCBI) 7124
ENSEMBL Gene IDENSG00000232810
RRIDAB_3671322
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01375
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.

Protocols

Product Specific Protocols
WB protocol for TNF-alpha antibody 83721-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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