TNF-alpha Monoclonal Matched Antibody Pair, PBS Only
Host / Isotype
Capture: Mouse / IgG1
Detection: Mouse / IgG1
Reactivity
Capture: human
Detection: human
Applications
Sandwich ELISA
Range
Sandwich ELISA: 0.020-5 ng/mL
Validation Data Gallery
Tested Applications
Sandwich ELISA
Recommended dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
MP50093-1 targets TNF-alpha in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.
Range: 0.020-5 ng/mL (Sandwich ELISA);
Capture antibody: TNF alpha Monoclonal antibody, PBS Only (Capture) 68740-1-PBS (2E5G4). 100 μg. Concentration 1 mgl/ml.
Detection antibody: TNF-alpha Monoclonal antibody, PBS Only (Detector) 68740-2-PBS (1B2C2). 100 μg. Concentration 1 mgl/ml.
Alternative TNF-alpha matched antibody pairs: MP00702-1, MP00702-2, MP00702-3, MP00702-4
Matched pair protein standard: HZ-1014
Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.
Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)
Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.
| Catalog Number | 68740-1-PBS |
| Tested Reactivity | human |
| Immunogen | TNF alpha fusion protein HZ-1014 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Affinity | KD=2.43 x 10-9M KOff=7.28 x 10-5M KOn=3.00 x 104M |
| Full Name | tumor necrosis factor (TNF superfamily, member 2) |
| Synonyms | TNF, TNF-a, Intracellular domain 2, Intracellular domain 1, C-domain 2 |
| Gene symbol | TNF-alpha |
| Gene ID (NCBI) | 7124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |
| Catalog Number | 68740-2-PBS |
| Tested Reactivity | human |
| Immunogen | TNF-alpha fusion protein HZ-1014 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Affinity | KD=3.74 x 10-10M KOff=2.28 x 10-6M KOn=6.11 x 103M |
| Full Name | tumor necrosis factor (TNF superfamily, member 2) |
| Synonyms | TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1 |
| Gene symbol | TNF-alpha |
| Gene ID (NCBI) | 7124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |