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CoraLite®594-conjugated TNFR1/CD120a Monoclonal antibody
TNFR1/CD120a Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2A6E3
Cat no : CL594-60192
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human liver cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-60192 targets TNFR1/CD120a in IF-P applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TNFR1/CD120a fusion protein Ag16112 相同性解析による交差性が予測される生物種 |
| Full Name | tumor necrosis factor receptor superfamily, member 1A |
| Calculated molecular weight | 455 aa, 50 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC010140 |
| Gene symbol | TNFR1 |
| Gene ID (NCBI) | 7132 |
| RRID | AB_2919901 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 TNFR1/CD120a antibody CL594-60192 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
