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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNFR2 / TNFRSF1B Polyclonal antibody, PBS Only

TNFR2 / TNFRSF1B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28746-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TNFR2 / TNFRSF1B Polyclonal antibody (28746-1-AP)

    WB analysis using 28746-1-AP (same clone as 28746-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TNFR2 / TNFRSF1B Polyclonal antibody (28746-1-AP)

    IHC staining of human tonsillitis using 28746-1-AP (same clone as 28746-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TNFR2 / TNFRSF1B Polyclonal antibody (28746-1-AP)

    IHC staining of human tonsillitis using 28746-1-AP (same clone as 28746-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using TNFR2 / TNFRSF1B Polyclonal antibody (28746-1-AP)

    IHC staining of human spleen using 28746-1-AP (same clone as 28746-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using TNFR2 / TNFRSF1B Polyclonal antibody (28746-1-AP)

    IHC staining of human spleen using 28746-1-AP (same clone as 28746-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using TNFR2 / TNFRSF1B Polyclonal antibody (28746-1-AP)

    IF Staining of THP-1 using 28746-1-AP (same clone as 28746-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TNFR2 / TNFRSF1B antibody (28746-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28746-1-PBS targets TNFR2 / TNFRSF1B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNFR2 / TNFRSF1B fusion protein Ag30490 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 1B
Calculated molecular weight 48 kDa
Observed molecular weight 75 kDa, 65 kDa
GenBank accession numberBC052977
Gene symbol TNFR2
Gene ID (NCBI) 7133
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).