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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNIK Monoclonal antibody

TNIK Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E9H3

Cat no : 67948-1-Ig

Synonyms

TNIK, TRAF2 and NCK-interacting protein kinase, 1E9H3, EC:2.7.11.1, KIAA0551

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using TNIK Monoclonal antibody (67948-1-Ig)

    WB analysis of K-562 using 67948-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood platelets using TNIK Monoclonal antibody (67948-1-Ig)

    WB analysis of human peripheral blood platelets using 67948-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TNIK Monoclonal antibody (67948-1-Ig)

    IHC staining of human kidney using 67948-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TNIK Monoclonal antibody (67948-1-Ig)

    IHC staining of human kidney using 67948-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TNIK Monoclonal antibody (67948-1-Ig)

    IF Staining of A431 using 67948-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TNIK antibody (67948-1-Ig, Clone: 1E9H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using TNIK Monoclonal antibody (67948-1-Ig)

    FC experiment of A431 using 67948-1-Ig

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TNIK (67948-1-Ig, Clone:1E9H3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inhuman peripheral blood platelets, K-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

67948-1-Ig targets TNIK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TNIK fusion protein Ag30710 相同性解析による交差性が予測される生物種
Full Name TRAF2 and NCK interacting kinase
Calculated molecular weight 155 kDa
Observed molecular weight155 kDa
GenBank accession numberBC055427
Gene symbol TNIK
Gene ID (NCBI) 23043
RRIDAB_2918700
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Traf2 and NcK interacting kinase (TNiK) contains serine-threonine kinase and scaffold domains and has been implicated in cell proliferation and glutamate receptor regulation in vitro.

Protocols

Product Specific Protocols
WB protocol for TNIK antibody 67948-1-IgDownload protocol
IHC protocol for TNIK antibody 67948-1-IgDownload protocol
IF protocol for TNIK antibody 67948-1-IgDownload protocol
FC protocol for TNIK antibody 67948-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Mol Biosci

Comprehensive analysis of senescence-related genes and immune infiltration in intervertebral disc degeneration: a meta-data approach utilizing bulk and single-cell RNA sequencing data

Authors - Ya-Jun Deng
View Article