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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNIK Monoclonal antibody, PBS Only

TNIK Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E9H3

Cat no : 67948-1-PBS

Synonyms

TNIK, TRAF2 and NCK-interacting protein kinase, 1E9H3, EC:2.7.11.1, KIAA0551

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using TNIK Monoclonal antibody (67948-1-Ig)

    WB analysis of K-562 using 67948-1-Ig (same clone as 67948-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using TNIK Monoclonal antibody (67948-1-Ig)

    WB analysis of human peripheral blood platelets using 67948-1-Ig (same clone as 67948-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TNIK Monoclonal antibody (67948-1-Ig)

    IHC staining of human kidney using 67948-1-Ig (same clone as 67948-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TNIK Monoclonal antibody (67948-1-Ig)

    IHC staining of human kidney using 67948-1-Ig (same clone as 67948-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TNIK Monoclonal antibody (67948-1-Ig)

    IF Staining of A431 using 67948-1-Ig (same clone as 67948-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TNIK antibody (67948-1-Ig, Clone: 1E9H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using TNIK Monoclonal antibody (67948-1-Ig)

    FC experiment of A431 using 67948-1-Ig (same clone as 67948-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TNIK (67948-1-Ig, Clone:1E9H3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67948-1-PBS targets TNIK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TNIK fusion protein Ag30710 相同性解析による交差性が予測される生物種
Full Name TRAF2 and NCK interacting kinase
Calculated molecular weight 155 kDa
GenBank accession numberBC055427
Gene symbol TNIK
Gene ID (NCBI) 23043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.