Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-13504 targets TNNC1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TNNC1 fusion protein Ag4339 相同性解析による交差性が予測される生物種 |
| Full Name | troponin C type 1 (slow) |
| Calculated molecular weight | 161 aa, 18 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC030244 |
| Gene Symbol | TNNC1 |
| Gene ID (NCBI) | 7134 |
| RRID | AB_3672568 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P63316 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. TnC plays an important role in excitation-contraction coupling and is the primary target of compounds such as levosimendan that augment Ca2+ responsiveness of the thin filament.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TNNC1 antibody CL488-13504 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |