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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNS4 Polyclonal antibody (11580-1-AP)

    WB analysis using 11580-1-AP (same clone as 11580-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11580-1-AP (TNS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11580-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using TNS4 Polyclonal antibody (11580-1-AP)

    WB analysis of human placenta using 11580-1-AP (same clone as 11580-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11580-1-AP (TNS4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11580-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TNS4 Polyclonal antibody (11580-1-AP)

    IHC staining of human liver cancer using 11580-1-AP (same clone as 11580-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11580-1-AP (TNS4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11580-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TNS4 Polyclonal antibody (11580-1-AP)

    IHC staining of human liver cancer using 11580-1-AP (same clone as 11580-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11580-1-AP (TNS4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11580-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TNS4 Polyclonal antibody (11580-1-AP)

    IF Staining of A549 using 11580-1-AP (same clone as 11580-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11580-1-AP (TNS4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11580-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TNS4 Polyclonal antibody, PBS Only

TNS4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11580-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11580-1-PBS targets TNS4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNS4 fusion protein Ag2169 相同性解析による交差性が予測される生物種
Full Name tensin 4
Calculated molecular weight 715 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC018706
Gene Symbol TNS4
Gene ID (NCBI) 84951
RRIDAB_10640582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZW8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TNS4 (Tensin-4), also known as COOH-terminus tensin-like protein, is a distinctive member with the smallest molecular mass and lacks the N-terminal common region found in other family members. TNS4 plays a crucial role in CRC tumorigenesis, and that suppression of TNS4 would be an effective therapeutic strategy in treating a subset of cetuximab-refractory CRC patients including KRAS activating mutations (PMID: 31409052). TNS4 has been implicated in numerous vital biological processes, including proliferation, migration, cell adhesion, and apoptosis (PMID: 38164166).