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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TOE1 Monoclonal antibody

TOE1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C10H7

Cat no : 67829-1-Ig

Synonyms

FLJ13949, Target of EGR1 protein 1, TOE1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOE1 Monoclonal antibody (67829-1-Ig)

    WB analysis using 67829-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67829-1-Ig (TOE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TOE1 Monoclonal antibody (67829-1-Ig)

    IHC staining of human colon cancer using 67829-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TOE1 Monoclonal antibody (67829-1-Ig)

    IHC staining of human colon cancer using 67829-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TOE1 Monoclonal antibody (67829-1-Ig)

    IHC staining of human colon cancer using 67829-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TOE1 Monoclonal antibody (67829-1-Ig)

    IHC staining of human colon cancer using 67829-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOE1 Monoclonal antibody (67829-1-Ig)

    IF Staining of HepG2 using 67829-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOE1 antibody (67829-1-Ig, Clone: 2C10H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67829-1-Ig targets TOE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TOE1 fusion protein Ag28982 相同性解析による交差性が予測される生物種
Full Name target of EGR1, member 1 (nuclear)
Calculated molecular weight 510 aa, 57 kDa
Observed molecular weight57 kDa
GenBank accession numberBC009364
Gene symbol TOE1
Gene ID (NCBI) 114034
RRIDAB_2918591
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.

Protocols

Product Specific Protocols
WB protocol for TOE1 antibody 67829-1-IgDownload protocol
IHC protocol for TOE1 antibody 67829-1-IgDownload protocol
IF protocol for TOE1 antibody 67829-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols