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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TOE1 Monoclonal antibody, PBS Only

TOE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C10H7

Cat no : 67829-1-PBS

Synonyms

FLJ13949, Target of EGR1 protein 1, TOE1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOE1 Monoclonal antibody (67829-1-Ig)

    WB analysis using 67829-1-Ig (same clone as 67829-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67829-1-Ig (TOE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TOE1 Monoclonal antibody (67829-1-Ig)

    IHC staining of human colon cancer using 67829-1-Ig (same clone as 67829-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TOE1 Monoclonal antibody (67829-1-Ig)

    IHC staining of human colon cancer using 67829-1-Ig (same clone as 67829-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TOE1 Monoclonal antibody (67829-1-Ig)

    IHC staining of human colon cancer using 67829-1-Ig (same clone as 67829-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TOE1 Monoclonal antibody (67829-1-Ig)

    IHC staining of human colon cancer using 67829-1-Ig (same clone as 67829-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOE1 Monoclonal antibody (67829-1-Ig)

    IF Staining of HepG2 using 67829-1-Ig (same clone as 67829-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOE1 antibody (67829-1-Ig, Clone: 2C10H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67829-1-PBS targets TOE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TOE1 fusion protein Ag28982 相同性解析による交差性が予測される生物種
Full Name target of EGR1, member 1 (nuclear)
Calculated molecular weight 510 aa, 57 kDa
Observed molecular weight57 kDa
GenBank accession numberBC009364
Gene symbol TOE1
Gene ID (NCBI) 114034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.