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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TOE1 Polyclonal antibody

TOE1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16203

Synonyms

Target of EGR1 protein 1, TOE1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated TOE1 Polyclonal anti (CL488-16203)

    IF Staining of HeLa using CL488-16203

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TOE1 antibody (CL488-16203) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated TOE1 Polyclonal anti (CL488-16203)

    FC experiment of HeLa using CL488-16203

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated TOE1 Polyclonal antibody (CL488-16203)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16203 targets TOE1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOE1 fusion protein Ag9172 相同性解析による交差性が予測される生物種
Full Name target of EGR1, member 1 (nuclear)
Calculated molecular weight 510 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC009364
Gene symbol TOE1
Gene ID (NCBI) 114034
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TOE1 antibody CL488-16203Download protocol
FC protocol for CL Plus 488 TOE1 antibody CL488-16203Download protocol
Standard Protocols
Click here to view our Standard Protocols