CoraLite® Plus 488-conjugated Tom22 Monoclonal antibody
Tom22 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1C2B11
Cat no : CL488-66562
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66562 targets Tom22 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Tom22 fusion protein Ag1797 相同性解析による交差性が予測される生物種 |
Full Name | translocase of outer mitochondrial membrane 22 homolog (yeast) |
Calculated molecular weight | 15 kDa |
Observed molecular weight | 22 kDa |
GenBank accession number | BC009363 |
Gene symbol | TOMM22/Tom22 |
Gene ID (NCBI) | 56993 |
RRID | AB_2883356 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TOMM22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises the 22 kDa protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Tom22 antibody CL488-66562 | Download protocol |
FC protocol for CL Plus 488 Tom22 antibody CL488-66562 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |