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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated TOMM40 Polyclonal an (CL488-18409)

    IF Staining of HepG2 using CL488-18409

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TOMM40 antibody (CL488-18409) at dilution of 1:200, CL594-phalloidin (red).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TOMM40 Polyclonal antibody

TOMM40 Polyclonal Antibody for IF/ICC
Cat No. CL488-18409

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

C19orf1, D19S1177E, p38.5, PER EC1, PEREC1, Protein Haymaker, TOM40, TOMM40

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18409 targets TOMM40 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOMM40 fusion protein Ag13065 相同性解析による交差性が予測される生物種
Full Name translocase of outer mitochondrial membrane 40 homolog (yeast)
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC017224
Gene Symbol TOMM40
Gene ID (NCBI) 10452
RRIDAB_2923744
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO96008
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The translocase of outer mitochondria membrane 40 (TOMM40, also known as TOM40), located in the center of the TOM complex, is a channel-forming subunit of translocase. It can facilitate the fluid movement of preproteins into the mitochondria by associating with TOMM20. TOMM40 plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PMID: 31206022). TOMM40 has been reported to be associated with late-onset neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TOMM40 antibody CL488-18409Download protocol
Standard Protocols
Click here to view our Standard Protocols