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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TP63 Monoclonal antibody

TP63 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4D6G12

Cat no : 60332-2-Ig

Synonyms

p63, CUSP, tumor protein p63, Tumor protein 63, Transformation-related protein 63

Validation Data Gallery

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  • Western Blot (WB) analysis of HaCaT cells using TP63 Monoclonal antibody (60332-2-Ig)

    WB analysis of HaCaT using 60332-2-Ig

    HaCaT cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using TP63 Monoclonal antibody (60332-2-Ig)

    WB analysis of A431 using 60332-2-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using TP63 Monoclonal antibody (60332-2-Ig)

    WB analysis of U-937 using 60332-2-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat skin tissue using TP63 Monoclonal antibody (60332-2-Ig)

    IHC staining of rat skin using 60332-2-Ig

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TP63 Monoclonal antibody (60332-2-Ig)

    IHC staining of human tonsillitis using 60332-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using TP63 Monoclonal antibody (60332-2-Ig)

    IHC staining of mouse skin using 60332-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TP63 Monoclonal antibody (60332-2-Ig)

    IHC staining of human tonsillitis using 60332-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TP63 Monoclonal antibody (60332-2-Ig)

    IHC staining of human prostate cancer using 60332-2-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHaCaT cells, A431 cells, U-937 cells
Positive IHC detected inrat skin tissue, human prostate cancer tissue, human tonsillitis tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

60332-2-Ig targets TP63 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TP63 fusion protein Ag2791 相同性解析による交差性が予測される生物種
Full Name tumor protein p63
Calculated molecular weight 680 aa, 77 kDa
Observed molecular weight70 kDa
GenBank accession numberBC039815
Gene symbol TP63
Gene ID (NCBI) 8626
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.

Protocols

Product Specific Protocols
WB protocol for TP63 antibody 60332-2-IgDownload protocol
IHC protocol for TP63 antibody 60332-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dose Response

Scutellarin Inhibits Glioblastoma Growth in a Dose-dependent Manner by Suppressing the p63 Signaling Pathway

Authors - Yongjie Chen
View Article