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Validation Data Gallery

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  • Western Blot (WB) analysis of HaCaT cells using p63 Monoclonal antibody (60332-2-Ig)

    WB analysis of HaCaT using 60332-2-Ig (same clone as 60332-2-PBS)

    HaCaT cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using p63 Monoclonal antibody (60332-2-Ig)

    WB analysis of A431 using 60332-2-Ig (same clone as 60332-2-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using p63 Monoclonal antibody (60332-2-Ig)

    WB analysis of U-937 using 60332-2-Ig (same clone as 60332-2-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using p63 Monoclonal antibody (60332-2-Ig)

    IHC staining of rat skin using 60332-2-Ig (same clone as 60332-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using p63 Monoclonal antibody (60332-2-Ig)

    IHC staining of human tonsillitis using 60332-2-Ig (same clone as 60332-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using p63 Monoclonal antibody (60332-2-Ig)

    IHC staining of mouse skin using 60332-2-Ig (same clone as 60332-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using p63 Monoclonal antibody (60332-2-Ig)

    IHC staining of human prostate cancer using 60332-2-Ig (same clone as 60332-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using p63 Monoclonal antibody (60332-2-Ig)

    IHC staining of human tonsillitis using 60332-2-Ig (same clone as 60332-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using p63 Monoclonal antibody (60332-2-Ig)

    IHC staining of human tonsillitis using 60332-2-Ig (same clone as 60332-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

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p63 Monoclonal antibody, PBS Only

p63 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60332-2-PBS
Clone No.4D6G12

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

TP63, tumor protein p63, Tumor protein 63, Transformation-related protein 63, TP 63

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60332-2-PBS targets p63 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen p63 fusion protein Ag2791 相同性解析による交差性が予測される生物種
Full Name tumor protein p63
Calculated molecular weight 680 aa, 77 kDa
Observed molecular weight70 kDa
GenBank accession numberBC039815
Gene Symbol TP63
Gene ID (NCBI) 8626
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H3D4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.