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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPMT Monoclonal antibody

TPMT Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D4B3

Cat no : 68251-1-Ig

Synonyms

Thiopurine methyltransferase, thiopurine S methyltransferase, TPMT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPMT Monoclonal antibody (68251-1-Ig)

    WB analysis using 68251-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68251-1-Ig (TPMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TPMT Monoclonal antibody (68251-1-Ig)

    IF Staining of HepG2 using 68251-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TPMT antibody (68251-1-Ig, Clone: 1D4B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, k-562 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68251-1-Ig targets TPMT in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TPMT fusion protein Ag28693 相同性解析による交差性が予測される生物種
Full Name thiopurine S-methyltransferase
Calculated molecular weight 28 kDa
Observed molecular weight32 kDa
GenBank accession numberBC005339
Gene symbol TPMT
Gene ID (NCBI) 7172
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The TPMT gene is localized to chromosome 6p22.3 and is approximately 34 kDa in length. It has an open reading frame (ORF) of 735 bp that encodes for a 245-amino acid peptide. The wild type TPMT allele, expressing the enzyme with normal activity. To date, there have been 30 variant TPMT alleles described (PMID: 22493988). Thiopurine compounds are commonly used in the treatment of childhood acute lymphoblastic leukemia, and as immunosuppressants following organ transplantation or for treatment of various autoimmune disorders. Thiopurine S-methyltransferase (TPMT) is required for detoxification, through S-methylation, of 6-thioguanine nucleotides (TGNs), a byproduct of thiopurine metabolism. Catalog#10682-1-AP is a rabbit polyclonal antibody raised against the full-length of human TPMT. TPMT has a predicted molecular weight of 28 kDa, however, as a result of its phosphorylation it separates on a SDS gel as a band of approximately 28-32 kDa.

Protocols

Product Specific Protocols
WB protocol for TPMT antibody 68251-1-IgDownload protocol
IF protocol for TPMT antibody 68251-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols