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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated TPPII Monoclonal antibody

TPPII Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5H6F11

Cat no : CL594-66017

Synonyms

FLJ40359, TPP 2, TPP II, TPP2, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated TPPII Monoclonal antibody (CL594-66017)

    IF Staining of HeLa using CL594-66017

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66017 (TPPII antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66017 is TPPII Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TPPII fusion protein Ag5296 相同性解析による交差性が予測される生物種
Full Name tripeptidyl peptidase II
Calculated molecular weight 1249 aa, 138 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC039905
Gene symbol TPPII
Gene ID (NCBI) 7174
RRIDAB_2883479
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

Protocols

Product Specific Protocols
IF protocol for CL594 TPPII antibody CL594-66017Download protocol
Standard Protocols
Click here to view our Standard Protocols