Validation Data Gallery
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Tested Applications
| Positive WB detected in | human kidney tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
66174-1-Ig targets TPSAB1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TPSAB1 fusion protein Ag4167 相同性解析による交差性が予測される生物種 |
| Full Name | tryptase alpha/beta 1 |
| Calculated molecular weight | 275 aa, 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC028059 |
| Gene Symbol | TPSAB1 |
| Gene ID (NCBI) | 7177 |
| RRID | AB_2881569 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15661 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TPSAB1(Tryptase alpha/beta-1) is also named as TPS1, TPS2, TPSB1 and belongs to the peptidase S1 family. It is the major neutral protease present in mast cells and secreted upon the coupled activation-degranulation response of this cell type. TPSAB1 has 2 isoforms produced by alternative splicing with the molecular weight of 31 kDa and 29 kDa. This full length protein has two glycosylation sites.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TPSAB1 antibody 66174-1-Ig | Download protocol |
| IHC protocol for TPSAB1 antibody 66174-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene Mast cells inhibit colorectal cancer development by inducing ER stress through secreting Cystatin C |