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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPSAB1 Monoclonal antibody, PBS Only (Capture)

TPSAB1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6A10G1

Cat no : 66174-1-PBS

Synonyms

TPSB1, TPS2, TPS1, MCP7, EC:3.4.21.59

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using TPSAB1 Monoclonal antibody (66174-1-Ig)

    WB analysis of human kidney using 66174-1-Ig (same clone as 66174-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66174-1-Ig (TPSAB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66174-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Monoclonal antibody (66174-1-Ig)

    IHC staining of human tonsillitis using 66174-1-Ig (same clone as 66174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66174-1-Ig (TPSAB1 antibody) at dilution of 1:5000 (under 10x lens) microwave oven heating mediated antigen retrieved with . This data was developed using the same antibody clone with 66174-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TPSAB1 Monoclonal antibody (66174-1-Ig)

    IHC staining of human tonsillitis using 66174-1-Ig (same clone as 66174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66174-1-Ig (TPSAB1 antibody) at dilution of 1:5000 (under 40x lens) microwave oven heating mediated antigen retrieved with . This data was developed using the same antibody clone with 66174-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50385-1

    Cytometric bead array standard curve of MP50385-1

    Cytometric bead array standard curve of MP50385-1, TPSAB1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66174-1-PBS. Detection antibody: 66174-2-PBS. Standard:Ag4167. Range: 3.125-200 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66174-1-PBS targets TPSAB1 as part of a matched antibody pair:

MP50385-1: 66174-1-PBS capture and 66174-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TPSAB1 fusion protein Ag4167 相同性解析による交差性が予測される生物種
Full Name tryptase alpha/beta 1
Calculated molecular weight 275 aa, 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC028059
Gene symbol TPSAB1
Gene ID (NCBI) 7177
RRIDAB_2881569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TPSAB1(Tryptase alpha/beta-1) is also named as TPS1, TPS2, TPSB1 and belongs to the peptidase S1 family. It is the major neutral protease present in mast cells and secreted upon the coupled activation-degranulation response of this cell type. TPSAB1 has 2 isoforms produced by alternative splicing with the molecular weight of 31 kDa and 29 kDa. This full length protein has two glycosylation sites.