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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated TPT1 Monoclonal antibody

TPT1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1G6A10

Cat no : CL555-66713

Synonyms

FLJ27337, Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite®555-conjugated TPT1 Monoclonal antibody (CL555-66713)

    IF Staining of HEK-293 using CL555-66713

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using CoraLite®555 TPT1 antibody (CL555-66713, Clone: 1G6A10 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66713 targets TPT1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TPT1 fusion protein Ag27651 相同性解析による交差性が予測される生物種
Full Name tumor protein, translationally-controlled 1
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC003352
Gene symbol TPT1
Gene ID (NCBI) 7178
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.

Protocols

Product Specific Protocols
IF protocol for CL555 TPT1 antibody CL555-66713Download protocol
Standard Protocols
Click here to view our Standard Protocols