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TPX2 Polyclonal antibody

Name: TPX2 Polyclonal antibody
Brand: Proteintech
SKU: 11741-1-AP

TPX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11741-1-AP

Synonyms

DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 11741-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11741-1-AP (TPX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11741-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11741-1-AP (TPX2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11741-1-AP
IP result of anti-TPX2 (IP:11741-1-AP, 4ug; Detection:11741-1-AP 1:800) with HeLa cells lysate 880ug.
IHC staining of human colon cancer using 11741-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11741-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11741-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11741-1-AP (TPX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11741-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11741-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11741-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11741-1-AP (TPX2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 11741-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPX2 antibody (11741-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HeLa using 11741-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11741-1-AP (TPX2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 11741-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, K-562 cells, HeLa cells, SMMC-7721 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 16 publications below
IHC	See 4 publications below
IF	See 6 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below

Product Information

11741-1-AP targets TPX2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TPX2 fusion protein Ag2334 相同性解析による交差性が予測される生物種
Full Name	TPX2, microtubule-associated, homolog (Xenopus laevis)
Calculated molecular weight	747 aa, 86 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC020207
Gene symbol	TPX2
Gene ID (NCBI)	22974
RRID	AB_2208895
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).

Protocols

Product Specific Protocols
WB protocol for TPX2 antibody 11741-1-AP	Download protocol
IHC protocol for TPX2 antibody 11741-1-AP	Download protocol
IF protocol for TPX2 antibody 11741-1-AP	Download protocol
IP protocol for TPX2 antibody 11741-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB, IF	J Cell Biol WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin. Authors - Junjie Huang View Article
mouse	WB,IF	EMBO Rep Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis Authors - Jia-Qian Ju View Article
human	WB,IF	BMC Biol CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment. Authors - Haining Zhou View Article
mouse	WB	J Cell Sci Loss of protein phosphatase 6 in oocytes causes failure of meiosis II exit and impaired female fertility. Authors - Meng-Wen Hu View Article
human	IF	J Cell Sci Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation. Authors - Haijing Guo View Article
human	IHC	Front Physiol Dysfunction of Sister Chromatids Separation Promotes Progression of Hepatocellular Carcinoma According to Analysis of Gene Expression Profiling. Authors - Baozhen Sun View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TPX2 Polyclonal antibody

TPX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 11741-1-AP

WB analysis of K-562 using 11741-1-AP

IP experiment of HeLa using 11741-1-AP

IHC staining of human colon cancer using 11741-1-AP

IHC staining of human colon cancer using 11741-1-AP

IHC staining of human prostate cancer using 11741-1-AP

IHC staining of human breast cancer using 11741-1-AP

IHC staining of human breast cancer using 11741-1-AP

IF Staining of HeLa using 11741-1-AP

IF Staining of HepG2 using 11741-1-AP

IF Staining of HeLa using 11741-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.

Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis

CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment.

Loss of protein phosphatase 6 in oocytes causes failure of meiosis II exit and impaired female fertility.

Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation.

Dysfunction of Sister Chromatids Separation Promotes Progression of Hepatocellular Carcinoma According to Analysis of Gene Expression Profiling.