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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated TPX2 Polyclonal anti (CL488-11741)

    IF Staining of HepG2 using CL488-11741

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TPX2 antibody (CL488-11741) at dilution of 1:200, CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated TPX2 Polyclonal anti (CL488-11741)

    IF Staining of HeLa using CL488-11741

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TPX2 antibody (CL488-11741) at dilution of 1:200, CL594-Phalloidin (red).

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  • KD/KO Validated

CoraLite® Plus 488-conjugated TPX2 Polyclonal antibody

TPX2 Polyclonal Antibody for IF/ICC
Cat No. CL488-11741

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11741 targets TPX2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPX2 fusion protein Ag2334 相同性解析による交差性が予測される生物種
Full Name TPX2, microtubule-associated, homolog (Xenopus laevis)
Calculated molecular weight 747 aa, 86 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC020207
Gene Symbol TPX2
Gene ID (NCBI) 22974
RRIDAB_3672510
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULW0
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TPX2 antibody CL488-11741Download protocol
Standard Protocols
Click here to view our Standard Protocols