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- KD/KO Validated
TRAF4 Monoclonal antibody
TRAF4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, pig and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2C9A11
Cat no : 66755-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, THP-1 cells, U-937 cells, NCCIT cells, human brain tissue, pig brain tissue, HEK-293 cells, Ramos cells, Daudi cells, Raji cells, pig spleen tissue |
| Positive IHC detected in | human urothelial carcinoma tissue, human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
66755-1-Ig targets TRAF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.
| Tested Reactivity | Human, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TRAF4 fusion protein Ag27221 相同性解析による交差性が予測される生物種 |
| Full Name | TNF receptor-associated factor 4 |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 50-54 kDa |
| GenBank accession number | BC001769 |
| Gene symbol | TRAF4 |
| Gene ID (NCBI) | 9618 |
| RRID | AB_2882101 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The tumor necrosis factor receptor-associated factor (TRAF) family, consisting of seven members (TRAF1-7), is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor superfamily. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas. TRAF4 is required during embryogenesis in key biological processes including the formation of the trachea, the development of the axial skeleton, and the closure of the neural tube. TRAF4 deficiency leads to severe developmental alterations.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAF4 antibody 66755-1-Ig | Download protocol |
| IHC protocol for TRAF4 antibody 66755-1-Ig | Download protocol |
| IF protocol for TRAF4 antibody 66755-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci TRAF4 Promotes the Proliferation of Glioblastoma by Stabilizing SETDB1 to Activate the AKT Pathway
| ||
Anticancer Drugs High expression of TRAF4 predicts poor prognosis in tamoxifen-treated breast cancer and promotes tamoxifen resistance.
| ||
Adv Sci (Weinh) TRAF4-Mediated LAMTOR1 Ubiquitination Promotes mTORC1 Activation and Inhibits the Inflammation-Induced Colorectal Cancer Progression
| ||
