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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAF4 Monoclonal antibody (66755-1-Ig)

    WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TRAF4 Monoclonal antibody (66755-1-Ig)

    WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TRAF4 Monoclonal antibody (66755-1-Ig)

    WB analysis using 66755-1-Ig (same clone as 66755-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using TRAF4 Monoclonal antibody (66755-1-Ig)

    WB analysis of THP-1 using 66755-1-Ig (same clone as 66755-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using TRAF4 Monoclonal antibody (66755-1-Ig)

    IHC staining of human urothelial carcinoma using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using TRAF4 Monoclonal antibody (66755-1-Ig)

    IHC staining of human urothelial carcinoma using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRAF4 Monoclonal antibody (66755-1-Ig)

    IHC staining of human lung cancer using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRAF4 Monoclonal antibody (66755-1-Ig)

    IHC staining of human lung cancer using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRAF4 Monoclonal antibody (66755-1-Ig)

    IHC staining of human lung cancer using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRAF4 Monoclonal antibody (66755-1-Ig)

    IHC staining of human lung cancer using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRAF4 Monoclonal antibody (66755-1-Ig)

    IHC staining of human breast cancer using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRAF4 Monoclonal antibody (66755-1-Ig)

    IHC staining of human breast cancer using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAF4 Monoclonal antibody (66755-1-Ig)

    IF Staining of HeLa using 66755-1-Ig (same clone as 66755-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66755-1-Ig (TRAF4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66755-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TRAF4 Monoclonal antibody, PBS Only

TRAF4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66755-1-PBS
Clone No.2C9A11

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CART1, MLN 62, MLN62, RING finger protein 83, RNF83, TRAF4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66755-1-PBS targets TRAF4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAF4 fusion protein Ag27221 相同性解析による交差性が予測される生物種
Full Name TNF receptor-associated factor 4
Calculated molecular weight 54 kDa
Observed molecular weight 50-54 kDa
GenBank accession numberBC001769
Gene Symbol TRAF4
Gene ID (NCBI) 9618
RRIDAB_2882101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BUZ4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The tumor necrosis factor receptor-associated factor (TRAF) family, consisting of seven members (TRAF1-7), is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor superfamily. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas. TRAF4 is required during embryogenesis in key biological processes including the formation of the trachea, the development of the axial skeleton, and the closure of the neural tube. TRAF4 deficiency leads to severe developmental alterations.