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TRAP1 Monoclonal antibody

TRAP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1H5D4

Cat no : 67693-1-Ig

Synonyms

TNFR associated protein 1, HSPC5, HSP90L, HSP75, HSP 75

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAP1 Monoclonal antibody (67693-1-Ig)

    WB analysis using 67693-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67693-1-Ig (TRAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TRAP1 Monoclonal antibody (67693-1-Ig)

    WB analysis of HeLa using 67693-1-Ig

    WB result of TRAP1 antibody (67693-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAP1 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of K-562 cells using TRAP1 Monoclonal antibody (67693-1-Ig)

    IP experiment of K-562 using 67693-1-Ig

    IP result of anti-TRAP1 (IP:67693-1-Ig, 5ug; Detection:67693-1-Ig 1:1000) with K-562 cells lysate 1760 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRAP1 Monoclonal antibody (67693-1-Ig)

    IHC staining of mouse brain using 67693-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67693-1-Ig (TRAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAP1 Monoclonal antibody (67693-1-Ig)

    IF Staining of HeLa using 67693-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (67693-1-Ig, Clone: 1H5D4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAP1 Monoclonal antibody (67693-1-Ig)

    IF Staining of HeLa using 67693-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (67693-1-Ig, Clone: 1H5D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67693-1-Ig targets TRAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAP1 fusion protein Ag0400 相同性解析による交差性が予測される生物種
Full Name TNF receptor-associated protein 1
Calculated molecular weight 80 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC000406
Gene symbol TRAP1
Gene ID (NCBI) 10131
RRIDAB_2882886
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).

Protocols

Product Specific Protocols
WB protocol for TRAP1 antibody 67693-1-IgDownload protocol
IHC protocol for TRAP1 antibody 67693-1-IgDownload protocol
IF protocol for TRAP1 antibody 67693-1-IgDownload protocol
IP protocol for TRAP1 antibody 67693-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Virol Sin

Hsp90 β is Critical for the Infection of Severe Fever with Thrombocytopenia Syndrome Virus

Authors - Bo Wang
View Article