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  • Featured Product
  • KD/KO Validated

TRAP1 Monoclonal antibody, PBS Only

TRAP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H5D4

Cat no : 67693-1-PBS

Synonyms

TNFR associated protein 1, HSPC5, HSP90L, HSP75, HSP 75

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAP1 Monoclonal antibody (67693-1-Ig)

    WB analysis using 67693-1-Ig (same clone as 67693-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67693-1-Ig (TRAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TRAP1 Monoclonal antibody (67693-1-Ig)

    WB analysis of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

    WB result of TRAP1 antibody (67693-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAP1 transfected HeLa cells. This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using TRAP1 Monoclonal antibody (67693-1-Ig)

    IP experiment of K-562 using 67693-1-Ig (same clone as 67693-1-PBS)

    IP result of anti-TRAP1 (IP:67693-1-Ig, 5ug; Detection:67693-1-Ig 1:1000) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRAP1 Monoclonal antibody (67693-1-Ig)

    IHC staining of mouse brain using 67693-1-Ig (same clone as 67693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67693-1-Ig (TRAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAP1 Monoclonal antibody (67693-1-Ig)

    IF Staining of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (67693-1-Ig, Clone: 1H5D4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAP1 Monoclonal antibody (67693-1-Ig)

    IF Staining of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (67693-1-Ig, Clone: 1H5D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67693-1-PBS targets TRAP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAP1 fusion protein Ag0400 相同性解析による交差性が予測される生物種
Full Name TNF receptor-associated protein 1
Calculated molecular weight 80 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC000406
Gene symbol TRAP1
Gene ID (NCBI) 10131
RRIDAB_2882886
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).