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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAPA/SSR1 Monoclonal antibody

TRAPA/SSR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1F2D7

Cat no : 67925-1-Ig

Synonyms

DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAPA/SSR1 Monoclonal antibody (67925-1-Ig)

    WB analysis using 67925-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TRAPA/SSR1 Monoclonal antibody (67925-1-Ig)

    IHC staining of human ovary tumor using 67925-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TRAPA/SSR1 Monoclonal antibody (67925-1-Ig)

    IHC staining of human ovary tumor using 67925-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using TRAPA/SSR1 Monoclonal antibody (67925-1-Ig)

    IF Staining of human ovary tumor using 67925-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using TRAPA/SSR1 antibody (67925-1-Ig, Clone: 1F2D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman ovary tumor tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67925-1-Ig targets TRAPA/SSR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAPA/SSR1 fusion protein Ag30832 相同性解析による交差性が予測される生物種
Full Name signal sequence receptor, alpha
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC007710
Gene symbol TRAPA
Gene ID (NCBI) 6745
RRIDAB_2918677
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

Protocols

Product Specific Protocols
WB protocol for TRAPA/SSR1 antibody 67925-1-IgDownload protocol
IHC protocol for TRAPA/SSR1 antibody 67925-1-IgDownload protocol
IF protocol for TRAPA/SSR1 antibody 67925-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols