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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated TRAPA/SSR1 Monoclonal antibody

TRAPA/SSR1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F2D7

Cat no : CL488-67925

Synonyms

DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using CoraLite® Plus 488-conjugated TRAPA/SSR1 Monoclona (CL488-67925)

    IF Staining of human ovary tumor using CL488-67925

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 TRAPA/SSR1 antibody (CL488-67925, Clone: 1F2D7 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using CoraLite® Plus 488-conjugated TRAPA/SSR1 Monoclona (CL488-67925)

    IF Staining of human ovary tumor using CL488-67925

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 TRAPA/SSR1 antibody (CL488-67925, Clone: 1F2D7 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman ovary tumor tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67925 targets TRAPA/SSR1 in IF-P applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAPA/SSR1 fusion protein Ag30832 相同性解析による交差性が予測される生物種
Full Name signal sequence receptor, alpha
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC007710
Gene symbol TRAPA
Gene ID (NCBI) 6745
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TRAPA/SSR1 antibody CL488-67925Download protocol
Standard Protocols
Click here to view our Standard Protocols