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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TRAPPC4 Recombinant antibody (84119-3-RR)

    WB analysis of MCF-7 using 84119-3-RR (same clone as 84119-3-PBS)

    WB result of TRAPPC4 antibody (84119-3-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC4 transfected MCF-7 cells. This data was developed using the same antibody clone with 84119-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TRAPPC4 Recombinant antibody (84119-3-RR)

    WB analysis using 84119-3-RR (same clone as 84119-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84119-3-RR (TRAPPC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84119-3-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HL-60 cells using TRAPPC4 Recombinant antibody (84119-3-RR)

    IP experiment of HL-60 using 84119-3-RR (same clone as 84119-3-PBS)

    IP result of anti-TRAPPC4 (IP:84119-3-RR, 4ug; Detection:84119-3-RR 1:3000) with HL-60 cells lysate 2240 ug. This data was developed using the same antibody clone with 84119-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01030-2

    Cytometric bead array standard curve of MP01030-2

    Cytometric bead array standard curve of MP01030-2, TRAPPC4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84119-4-PBS. Detection antibody: 84119-3-PBS. Standard: Ag2737. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01030-3

    Cytometric bead array standard curve of MP01030-3

    Cytometric bead array standard curve of MP01030-3, TRAPPC4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84119-1-PBS. Detection antibody: 84119-3-PBS. Standard: Ag2737. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84119-3-RR

    Affinity and Kinetic Characterization of 84119-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84119-3-RR against Human TRAPPC4 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

TRAPPC4 Recombinant antibody, PBS Only (Detector)

TRAPPC4 Uni-rAbTM Recombinant Antibody for WB, IP, Cytometric bead array, Indirect ELISA
Cat No. 84119-3-PBS
Clone No.241158C11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Cytometric bead array, Indirect ELISA

Synonyms

SBDN, PTD009, HSPC172, CGI-104, CGI 104

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84119-3-PBS targets TRAPPC4 as part of a matched antibody pair:

MP01030-2: 84119-4-PBS capture and 84119-3-PBS detection (validated in Cytometric bead array)

MP01030-3: 84119-1-PBS capture and 84119-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRAPPC4 fusion protein Ag2737 相同性解析による交差性が予測される生物種
Full Name trafficking protein particle complex 4
Calculated molecular weight 219 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC010866
Gene Symbol TRAPPC4
Gene ID (NCBI) 51399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y296
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).