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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAPPC4 Recombinant antibody

TRAPPC4 Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

241158C11

Cat no : 84119-3-RR

Synonyms

SBDN, PTD009, HSPC172, CGI-104, CGI 104

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAPPC4 Recombinant antibody (84119-3-RR)

    WB analysis using 84119-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84119-3-RR (TRAPPC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HL-60 cells using TRAPPC4 Recombinant antibody (84119-3-RR)

    IP experiment of HL-60 using 84119-3-RR

    IP result of anti-TRAPPC4 (IP:84119-3-RR, 4ug; Detection:84119-3-RR 1:3000) with HL-60 cells lysate 2240 ug.

  • Affinity and Kinetic Characterization of 84119-3-RR

    Affinity and Kinetic Characterization of 84119-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84119-3-RR against Human TRAPPC4 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHCT 116 cells, HL-60 cells, RAW 264.7 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84119-3-RR is TRAPPC4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRAPPC4 fusion protein Ag2737 相同性解析による交差性が予測される生物種
Full Name trafficking protein particle complex 4
Calculated molecular weight 219 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC010866
Gene symbol TRAPPC4
Gene ID (NCBI) 51399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).

Protocols

Product Specific Protocols
WB protocol for TRAPPC4 antibody 84119-3-RRDownload protocol
IP protocol for TRAPPC4 antibody 84119-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols