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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated TRIB2 Monoclonal antibody

TRIB2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A10A6

Cat no : CL488-67171

Synonyms

TRB2, TRB 2, GS3955, FLJ57420, C5FW

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated TRIB2 Monoclonal ant (CL488-67171)

    IF Staining of A431 using CL488-67171

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 TRIB2 antibody (CL488-67171, Clone: 1A10A6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated TRIB2 Monoclonal ant (CL488-67171)

    FC experiment of HeLa using CL488-67171

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TRIB2 (CL488-67171, Clone:1A10A6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67171 targets TRIB2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIB2 fusion protein Ag7501 相同性解析による交差性が予測される生物種
Full Name tribbles homolog 2 (Drosophila)
Calculated molecular weight 39 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC002637
Gene symbol TRIB2
Gene ID (NCBI) 28951
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TRIB2 antibody CL488-67171Download protocol
Standard Protocols
Click here to view our Standard Protocols