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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TRIM14 Polyclonal antibody

TRIM14 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15742

Synonyms

Tripartite motif-containing protein 14, tripartite motif containing 14, TRIM, KIAA0129

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated TRIM14 Polyclonal an (CL488-15742)

    IF Staining of HeLa using CL488-15742

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 TRIM14 antibody (CL488-15742) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated TRIM14 Polyclonal an (CL488-15742)

    FC experiment of HeLa using CL488-15742

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Trim14 Polyclonal Antibody (CL488-15742)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15742 targets TRIM14 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM14 fusion protein Ag8158 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 14
Calculated molecular weight 442 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC006333
Gene symbol TRIM14
Gene ID (NCBI) 9830
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tripartite motif-containing 14(TRIM14) belongs to the TRIM protein family that contains 3 zinc-binding domains, a RING, a B-box type1 and a B-box type2 and plays key roles in cellular proliferation, differentiation, development, transformation, and immune response mechanisms . TRIM4 inhibits the transcriptional activity of SPI1 in a dose-dependent manner. It may participate in the early stages of differentiation of embryonic stem cells, leading to the formation of embryoid bodies.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TRIM14 antibody CL488-15742Download protocol
FC protocol for CL Plus 488 TRIM14 antibody CL488-15742Download protocol
Standard Protocols
Click here to view our Standard Protocols