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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated TRIM25 Monoclonal an (CL488-67314)

    IF Staining of HeLa using CL488-67314

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488-conjugated TRIM25 antibody (CL488-67314, Clone: 1H6G1 ) at dilution of 1:100.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated TRIM25 Monoclonal antibody

TRIM25 Monoclonal Antibody for IF/ICC
Cat No. CL488-67314
Clone No.1H6G1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67314 targets TRIM25 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIM25 fusion protein Ag29325 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 25
Calculated molecular weight 630 aa, 71 kDa
Observed molecular weight 68-71 kDa
GenBank accession numberBC016924
Gene Symbol TRIM25
Gene ID (NCBI) 7706
RRIDAB_2919449
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14258
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity (PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection (PMID: 17392790).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TRIM25 antibody CL488-67314Download protocol
Standard Protocols
Click here to view our Standard Protocols