Validation Data Gallery
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Tested Applications
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-10326 targets TRIM32 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRIM32 fusion protein Ag0401 相同性解析による交差性が予測される生物種 |
| Full Name | tripartite motif-containing 32 |
| Calculated molecular weight | 72 kDa |
| Observed molecular weight | 65-72 kDa |
| GenBank accession number | BC003154 |
| Gene Symbol | TRIM32 |
| Gene ID (NCBI) | 22954 |
| RRID | AB_3672438 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13049 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 TRIM32 antibody CL488-10326 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |