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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TRIM32 Polyclonal antibody

TRIM32 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10326

Synonyms

tripartite motif containing 32, RING-type E3 ubiquitin transferase TRIM32, HT2A, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM32

Validation Data Gallery

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  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated TRIM32 Polyclonal an (CL488-10326)

    FC experiment of A431 using CL488-10326

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TRIM32 (CL488-10326) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10326 targets TRIM32 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM32 fusion protein Ag0401 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 32
Calculated molecular weight 72 kDa
Observed molecular weight 65-72 kDa
GenBank accession numberBC003154
Gene symbol TRIM32
Gene ID (NCBI) 22954
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 TRIM32 antibody CL488-10326Download protocol
Standard Protocols
Click here to view our Standard Protocols