Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-10326 targets TRIM32 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TRIM32 fusion protein Ag0401 相同性解析による交差性が予測される生物種 |
Full Name | tripartite motif-containing 32 |
Calculated molecular weight | 72 kDa |
Observed molecular weight | 65-72 kDa |
GenBank accession number | BC003154 |
Gene Symbol | TRIM32 |
Gene ID (NCBI) | 22954 |
RRID | AB_3672438 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13049 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390).
Protocols
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 488 TRIM32 antibody CL488-10326 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |