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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM46 Recombinant antibody

TRIM46 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240557C2

Cat no : 83614-5-RR

Synonyms

Tripartite, fibronectin type-III and C-terminal SPRY motif protein, Tripartite motif-containing protein 46, tripartite motif containing 46, TRIFIC, GeneY

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TRIM46 Recombinant antibody (83614-5-RR)

    IF Staining of HepG2 using 83614-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRIM46 antibody (83614-5-RR, Clone: 240557C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of HeLa cells using TRIM46 Recombinant antibody (83614-5-RR)

    FC experiment of HeLa using 83614-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug TRIM46 Recombinant antibody (83614-5-RR, Clone:240557C2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83614-5-RR

    Affinity and Kinetic Characterization of 83614-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83614-5-RR against Human TRIM46 were performed. The affinity constant is 1.48 nM.

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83614-5-RR is TRIM46 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRIM46 fusion protein Ag15290 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 46
Calculated molecular weight 759 aa, 83 kDa
GenBank accession numberBC069416
Gene symbol TRIM46
Gene ID (NCBI) 80128
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).

Protocols

Product Specific Protocols
IF protocol for TRIM46 antibody 83614-5-RRDownload protocol
FC protocol for TRIM46 antibody 83614-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols